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    • 专利摘要:
    The present invention relates to a method for pest management using inhibitory repeat domain IRD 9 (Seq ID No.2) proteinase inhibitor showing enhanced inhibitory activity against the gut proteases of insects. More particularly, the present invention relates to a IRD 9 (Seq ID No.2) proteinase inhibitor from non-host plant
    公开号:AU2013207052A1
    申请号:U2013207052
    申请日:2013-01-07
    公开日:2014-07-24
    发明作者:Anirban Ghosh；Ashok Prabhakar GIRI；Vidya Shrikant GUPTA；Rajendra Ramchandra JOSHI；Rakesh Shamsunder JOSHI；Manasi MISHRA；Uddhavesh Bhaskar SONAVANE；Vaijayanti Abhijit TAMHANE
    申请人:Council of Scientific and Industrial Research CSIR；
    IPC主号:C07K14-435
    专利说明:
    WO 2013/102937 PCT/IN2013/000011 METHOD FOR EFFECTIVE MANAGEMENT OF HELICOVERPA ARMIGERA FIELD OF THE INVENTION The present invention relates to a method for effective management of Helicoverpa 5 armigera using inhibitory repeat domain IRD-9 of the Pin-II family proteinase inhibitors (CanPIs) having Seq ID No. 2. More particularly, the present invention relates to a IRD 9 (Seq ID No.2) proteinase inhibitor from non-host plant Capsicum annuum, which possesses significantly high insect protease inhibition activity against the gut proteases of Helicoverpa armigera. 10 BACKGROUND OF THE INVENTION Plants and insects have co-evolved in order to survive in their changing niches. Insects gradually adapt to take maximum nutritional benefit from the host while plants have evolved to defend themselves by up regulating the expression of defense related biochemicals (Bennett et al., 1994; Howe et al. 2008). In order to sustain on chemically 15 varied dietary content, insects display molecular flexibilities resulting in their modified gut enzyme complement and metabolism (Koiwa et al., 1997; Kessler and Ian T. Baldwin, 2002; Srinivasan et al. 2006; Dawkar et al., 2011). Helicoverpa armigera (Lepidoptera: Noctuidae), which is an agronomically important insect pest, has been widely studied for its polyphagy and adaptability on various host plants (Patankar et al., 20 2001; Sarate et al., 2011). Plant proteinase inhibitors (PIs) are ubiquitous in the plant kingdom and have been extensively studied as plant defense molecules which act by inhibiting hydrolytic enzymes from insect gut (Ryan,1990; Damle et al., 2005). Among various serine proteinase inhibitor (PI) families, Pin-II/Pot-Il family displays a remarkable structural 25 and functional diversity at gene and protein level (Johnson et al. 1989; McManas et al. 1994; Duan et al. 1996; Barta et al., 2001; Kong and Rangnathan 2008). Wound, insect and stress induced up regulation of these PIs clearly link their function to plant defense. 1 WO 2013/102937 PCT/IN2013/000011 Several studies have been undertaken in the past few decades using transgenic systems or in vivo assays. These studies positively correlate the insect defensive advantage offered by Pin-II PI expression in plants (Green and Ryan, 1972; Agrawal, 1998; Zavala et al., 2004a and b). Recently Pin-II PIs from Nicotiana alata expressed as transgene in 5 cotton and tested at the field level proved to enhance the productivity by 30% due to reduction in pest infestation (Dunse et al. 2010). In addition to the well-established defensive role, Pin-II PIs have been recently shown to have endogenous functions in plants which still remain to be fully elucidated (Sin and Chye, 2004; Wu et al., 2006; Johnson et al., 2007, Tamhane et al., 2009, Bezzi et al., 2010, Hartl et al., 2010). 10 Precursor proteins of Pin-II PIs consist of 1- to 8- inhibitory repeat domains (IRDs) connected by a protease sensitive linker, which upon cleavage releases IRD units. Each IRD is a peptide of around 50 amino acid with a- molecular mass of ~6 KDa. The amino acid sequences of inhibitory 'repeat domains show variations while the 8 cysteine residues and a single proline residue are almost conserved (Lee et al. 1999, Schirra et al. 15 2001, 2008 and 2010) throughout. Each IRD possesses a single active site either for trypsin or chymotrypsin inhibition based on the presence of lysine/ arginine or leucine at the P1 position respectively. The Pin-II precursor and/or the IRDs are both capable of simultaneously inhibiting several or single protease molecule respectively (Lee et al., 1999, Tamhane et al., 2007, Mishra et al., 2010). 20 Structure of Pin-II PIs, either 2 domain precursor or individual IRD(s) have been studied (Nielsen et al. 1994, Barrette Ng et al. 2003, Schirra and Craik, 2005). IRD shows a disordered loop containing the reactive site, a triple stranded beta sheet at its base and is anchored by four conserved disulfide bonds (C4-C41, C7-C25, C8-C37 and C14-C50) (Scanlon et al., 1999, Schirra et al., 2001, Schirra et al., 2008). Among the 25 four disulfide bonds, C8-C37 has been found to be very crucial for maintaining active conformation and hence inhibitory activity, whereas C4-C41 has important role in maintaining the flexibility of reactive loop (Schirra et al., 2010). Whereas selective loss 2 WO 2013/102937 PCT/IN2013/000011 of disulfide bond has evolutionary significance and leads to functional differentiation (Li et al, 2011). In a standard mechanism of protease inhibition by Pin-II PIs, the convex shaped reactive loop of inhibitor (P1 side chain) is recognized by concave active site (SI 5 binding pocket) of enzyme in a substrate like manner and plays a major role in the energetics of recognition (Czapifiska & Otlewski, 1999, Otlewski et al. 2001). Proteinase Inhibitor-proteinase interaction is further influenced by non-contact residues of the inhibitor by means of Van der Waals interaction and hydrogen (H) bonding. The structure of Pin-II IRDs or two domain PIs in complex with protease have been solved 10 (Greenblatt et al. 1989, Barrette Ng et al. 2003b). The structure displays the molecular framework of the PI-protease interaction. Whereas structure of unbound Pin-II inhibitor gives information about conformational flexibility of reactive loop and its role in modulation of proteinase binding efficiency (Barrette Ng et al. 2003a). Thermodynamic analysis of protease-proteinase inhibitor interaction shows that it is entropy driven 15 process (Otlewski et al., 2001). Different computational techniques like structure prediction, molecular dynamics and molecular docking studies have been used to study these interactions (Cui et al., 2005; Dunse et al., 2010). Earlier studies have shown that Pin-II PIs from Capsicum annuum (CanPIs) and their recombinant proteins show aiti -metabolic effects on the polyphagous and devastating' 20 insect pest H armigera by inhibiting larval growth and development (Tamhane et al., 2005; 2007). CanPIs interact with the gut proteases of the H armigera and are processed into their constituent IRDs (Mishra et al., 2010). 55 unique IRDs with amino acid variations in reactive loop and/or number of cysteine residues have been identified and characterized (Joshi et al., 2012; Mishra et al., 2012). Of these, the present 25 inventors have selected three CanPI IRDs on the basis of amino acid sequence variation and deviation from the presence of 8 conserved cysteine residues. 3 WO 2013/102937 PCT/IN2013/000011 The existing pest management strategies for controlling pests as described in the art are however putting very strong selective pressure on the insects thus leading to resistance. Therefore, there is a need to develop arena of effective and novel molecules, which can efficiently cause antibiosis of hazardous agricultural pest. 5 OBJECTS OF THE INVENTION Main object of the present invention is to provide a method for effective management of Helicoverpa armigera using inhibitory repeat domain IRD-9 of the Pin-II family proteinase inhibitors (CanPIs) having Seq ID No. 2. Another object of the present invention is to provide IRD 9 (Seq ID No.2) proteinase 10 inhibitor from non-host plant Capsicum annuum, which possesses significantly high insect protease inhibition activity against the gut proteases of Helicoverpa armigera. Yet another object of the present invention is to provide an effective pest management strategy for efficient antibiosis of hazardous agricultural pest. SUMMARY OF THE INVENTION 15 Accordingly, the present invention provide a method for effective management of Helicoverpa armigera using inhibitory repeat domain IRD-9 of the Pin-II family proteinase inhibitors (CanPIs) having Seq Id no. 2 comprising the steps: a. providing IRD -9 having Seq ID No. 2 from non-host plant Capsicum annuum, b. cloning of IRD-9 in Pichia pastoris (Yeast expression system) in pPIC-9 vector, 20 c. expressing and purifying IRD-9 protein, d. feeding IRD-9 protein to Helicoverpa armigera in artificial diet, e. calculating growth parameters for antibiosis effect of IRD-9. In another embodiment of the present invention, the method provides IRD-9 a variant of the Pin-II family proteinase inhibitors (CanPIs) characterized in having: 25 i. Molecular Weight: 5.8 Kd 4 WO 2013/102937 PCT/IN2013/000011 ii. Sequence length:- 50 amino acids iii. No. of cysteine residues: 6 iv. No. of disulfide bond: 2 v. Inhibition constant (Ki): -0.0022 mM 5 vi. Molecular interaction: reactive loop of IRD-9 form multiple hydrogen bonding with active site of target proteases. In still another embodiment of the present invention, use of IRD-9 a variant unit of the Pin-II family proteinase inhibitors (CanPIs), for effective pest management including efficient antibiosis of hazardous agricultural pest. 10 In still another embodiment of the present invention a composition for effective management of Helicoverpa armigera, comprising inhibitory repeat domain IRD-9 along with other excipients. In yet another embodiment of the present invention, use of IRD-9, a variant unit of the Pin-II family proteinase inhibitors (CanPIs) for effective pest management including 15 efficient antibiosis of hazardous agricultural pest. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1: Sequence and structural diversity of IRDs (A) The circular dendrogram of deduced AA sequences of IRDs. The three IRDs studied here are marked in colors as IRD-7: blue, IRD-9: red and IRD-12: magenta. 20 (B) Multiple sequence alignment of IRD-7, -9, -12 and Nicotiana alata trypsin inhibitor (NaTI) using DNASTAR and ClustalX2 software. Conserved cysteine residues are marked in yellow colour and the reactive loop region (residue 37-41) including the P1 residue is indicated with cyan colour. The number and position of cysteine residues are conserved in all except IRD-9 in which the cysteines at 7 th and 8 th positions are changed 25 to serine. The sequence of the reactive loops of IRD-7 and -9 is "CPKNC" whereas that of IRD- 12 and NaTI is "CPRNC". 5 WO 2013/102937 PCT/IN2013/000011 (C) Predicted structures of IRD-7 (Blue), -9 (Red) and -12 (Magenta). The reactive loop is marked with orange, cyan and violet colors while disulfide bonds with yellow. (D) The intramolecular hydrogen bond density was estimated in MD simulated structure of IRD-7, IRD-9 and IRD-12. Intra-molecular hydrogen'bonds were represented by blue 5 colour. spring structures. Figure 2: Inhibition studies for IRD-7, -9 and -12 (A) Purified protein preparations of IRDs; IRD-7, -9 and -12 show single band of approximately ~5.8 kDa on 15% Tricine-SDS-PAGE. (B) Inhibition of HGP activity with, 10 ptg of BApNA and Azocasein substrate. 10 (C) Estimation of IC 50 and Ki values by using inhibition of bovine trypsin with various concentrations of inhibitors and with substrate BApNA of concentration 1 mM. Figure 3: Biochemical characteristics of IRDs (A) Native-PAGE activity gel with equal units (i.e. 0.5 TIU) of a native and reduced sample of inhibitor IRD-7, IRD-9 and IRD-12 in consecutive lanes. Only normal 15 samples showed inhibitory activity, and not in the reduced state. (B) Inhibition activity of IRDs against HGP at different temperatures (C) In vitro stability of IRDs towards HGP. Equal HGPI units (0.5 Units) each of IRD 7, -9 and -12 were incubated with 1 HGP unit at 24'C for 5min (lane 2), 1 hr (lane 3) and the reaction mixtures were resolved on 15% native-PAGE gel. Each IRD without 20 HGP treatment (lane 1) was loaded as a control. The gels were processed for TI activity visualization by GXCT. IRD-9 shows higher intensities as compared with IRD-7 and, 12 in the presence of the HGP. (D) Comparative inhibition of HGP isoforms by different IRDs. Equal HGPI units of IRD-7, -9, -12 were incubated with HGP for 30min at 24'C. The above reaction 25 mixtures were then resolved on 8% native-PAGE. The gels were processed for protease 6 WO 2013/102937 PCT/IN2013/000011 activity visualization by GXCT. IRD-9 and -12 show inhibition of maximum HGP isoforms. (E) Synergistic inhibitory effect of different combinations of IRD-7, -9 and -12 using 0.5 mM of each protein. 5 Figure 4: The modelled H.armigera trypsin (grey) in complex with the predicted structures of the IRDs. The important residues at the interface of IRDs and trypsin in complexes of IRD-7 (orange), -9 (cyan), -12(violet) are shown separately in boxes. The models were obtained using a combination of homology modeling, loop prediction, and molecular dynamics. Thin dotted wheat colored lines represent hydrogen bonds. LYS 10 39H, ASN-40H in IRD-7, -9 and ARG-40H in IRD-12 form a number of important contacts with active site of HaTry. Figure 5: Correlation of theoretical AG with experimental AG showed regression coefficient. Figure 6: Inactivation of IRDs by serine modification using PMSF 15 Pseudo first-order plot for inactivation of IRDs by PMSF of concentration 5, 10 and 15 mM for 15, 30, 45 and 60 min (A) IRD-7 (B) IRD-9 and (C) IRD-12. Inset shows corresponding second order plot of pseudo-first order rate constants (K app) (min-) as a function of log (PMSF) concentration. Figure 7: In vivo efficacy of IRDs 20 Growth of H armigera larvae on diets containing IRDs. Eggs were hatched, and neonates were transferred to artificial diets containing 0.5 TUI of each IRD tested. (A) Larval weight gain was recorded every second day. The average weight of 25 larvae per treatment is shown. (B) The average size of larvae recorded on day 10. 25 (C) Average mortality rate of larvae fed on IRD containing diet. 7 WO 2013/102937 PCT/IN2013/000011 DETAILED DESCRIPTION OF THE INVENTION ABBREVIATIONS PIs: Plant proteinase inhibitors Pin-II family: Potato type inhibitor II family 5 IRDs: Inhibitory repeat domains CanPIs: Capsicum annuum proteinase inhibitors HaTry: Helicoverpa armigera trypsin Ki value: Inhibition Kinetics Constant The present invention relates to a method for effective management of Helicoverpa 10 armigera using inhibitory repeat domain IRD-9 of the Pin-II family proteinase inhibitors (CanPIs) having Seq ID No. 2. The novel plant proteinase inhibitor has tight binding affinities to the gut protease of Helicoverpa armigera and thus possesses significantly high protease inhibition activity. Capsicum annuum expresses a diversity of the Pin-II family proteinase inhibitors (CanPIs) comprising -6 kDa inhibitory repeat 15 domains (IRD) as their, basic functional unit. Each IRD contains eight conserved cysteines and forms a functional protein that has four disulphide bonds. The present inventors have isolated and characterized three variants IRDs of Pin-II Proteinase Inhibitors from non-host plant Capsicum annuum with six cysteine residues and also explored their interaction with Helicoverpa armigera trypsin (HaTry). The 20 three variants are IRD-7 (Seq ID No.1), IRD-9 (Seq ID No.2) and IRD-12 (Seq ID No.3). The intra molecular interactions like hydrogen bonds are significant for Pin-II PIs to retain and/or enhance their activity against target proteinase. Results obtained from activity gel show that IRD-9 binds significantly strong as compared to IRD-7 and IRD-12. In nature, frequency of IRD-9 (Seq ID No.2) is low 25 because it has strong synergistic effect which can help to elicit the inhibition by other IRDs and thus leads to their potentiation. 8 WO 2013/102937 PCT/IN2013/000011 Further, inhibition kinetics reveals that IRD-9, despite loss of some of the disulfide bonds, is a more potent proteinase inhibitor among the three selected IRDs. Molecular dynamic simulations reveal that serine residues in the place of cysteines at seventh and eighth positions of IRD-9 results in an increase in the density of intramolecular 5 hydrogen bonds and reactive site loop flexibility. Thus, due to its phenomenal activity and stability IRD-9 is used as a potential candidate for further application in the instant invention. The novel inhibitor of the current invention, IRD-9 has less number of disulphide bonds as compared to other known inhibitors from same class and also it has enhanced 0 inhibitory potential by several folds. In this inhibitor replacement of cysteine with hydrophilic amino acid provide stability for active conformation. The hydrophilic amino acids are selected from serine, threonine, tyrosine, asparagine, glutamine, aspartic acid, glutamic acid, lysine and arginine, particularly serine. The recombinant proteins and in silico analysis of three different IRDs namely, IRD-7, 15. IRD-12 (both with eight cysteine residues) and IRD-9 is carried out to study the functional significance of the variations. From inhibition kinetics with bovine trypsin and Helicoverpa armigera gut proteinase inhibition assays, it is found that IRD-9 has the highest inhibitory potential. Molecular modeling revealed that absence of two disulfide bonds in IRD-9 is compensated by higher density of intra-molecular H-bonds 20 which helps to retain its active conformation. IRD-9 displays enhanced flexibility of the reactive site loop leading to more contacts with the target enzyme. Biologicalrelevance of variations and functional differentiation of IRDs are also explored by in vitro simulation of natural PI-proteinase interaction using approaches like; combination of IRDs to tackle cocktail of insect gut proteinase, stability of IRDs and IF-MALDI-TOF 25 MS study. EXAMPLES The following examples are given by way of illustration and therefore should not be construed to limit the scope of the present invention. 9 WO 2013/102937 PCT/IN2013/000011 EXAMPLE 1 1. Materials and methods 1.1 Materials All reagents, enzymes and substrates were obtained from Sigma-Aldrich, St. Louis, 5 MO. Sterile plastics ware from Abdos, WB, India; expression vector pPIC9 and P. pastoris GS115 from Invitrogen, Carlsbad, CA, USA); Bradford reagent and electrophoresis reagents were from Bio-Rad Laboratories, Hercules, CA; X-ray films and Kodak 163 DA developer were purchased from Kodak, Chennai, India; HIC matrix i.e. Phenyl Sepharose and disposable PD-10 Desalting Columns were from GE 10 Healthcare Life Sciences, Uppsala, Sweden. 1.2 Selection, Cloning, Expression and Purification of IRD(s) Eighteen sequentially unique IRD(s) were identified from 21 CanPI genes, which were reported in our previous study (Tamhane et al., 2009). Phylogenetic analysis of these IRDs was carried out using MEGA5 software (http://www.megasoftware.net/). 15 Depending on sequence analysis IRD-7, -9 and -12 were selected for recombinant protein expression and further characterization. The mature peptide region of selected IRDs were cloned into expression vector pPIC9 for recombinant, extracellular expression in P. -pastoris GS115 and purified by hydrophobic interaction chromatography as described previously (Tamhane et al., 2005). The purified proteins 20 were quantified by Bradford reagent and checked for purity on 15% Tricine-SDS PAGE. 1.3 Inhibition assay and kinetics H armigera larvae were reared on artificial diet and complete gut tissue was dissected out from fourth instar larvae. H armigera gut proteases (HGP) were extracted from 2 25 gm of gut tissue by homogenizing in 0.2 M Glycine-NaOH buffer, pH 10.0 in 1:1 ratios (w/v) and kept at 4 'C for 2 h (Tamhane et al., 2005). The suspension was centrifuged at 13,OOOX g, 4 'C for 20 min and the resulting supernatant was used as a source of gut 10 WO 2013/102937 PCT/IN2013/000011 proteases of H armigera (HGP). Total proteolytic activity of 50 mM bovine trypsin/HGP and inhibition of their activity by IRDs (5 ig) was measured by Azocasein assays. Trypsin-like activity of the HGP and its inhibition by IRDs was also estimated through a BApNA assay using chromogenic substrate Benzoyl-L-arginyl p-nitroanilide 5 (BApNA). BApNA assays were performed as described previously (Tamhane et al., 2005; Tamhane et al., 2007) and HGPI units of all the IRDs were determined. HGP inhibitory (HGPI) unit is defined as the amount of protein that will inhibit 1 unit of HGP activity using BApNA as a substrate at 37'C, pH 7.8. Minimum three replicates of each experiment were performed. 10 Michaelis-Menten constant (Km) for trypsin was calculated by using various concentrations of BApNA substrate (1 to 5 mM), and then plotting double reciprocal curve with 1/(v) and 1/[S]. The kinetic properties of IRDs were analysed over a range of concentration of inhibitors (IpM to 1mM). IC 50 values for each inhibitor were calculated from the sigmoid curve indicating the best fit for the percentage inhibition 15 data obtained. The values of Ki values for each inhibitor were calculated directly from
    IC
    50 values using Cheng-Prusoff's classical equation (Copeland et al., 1995). (I 0) 1.4 Biochemical Characterization of IRD(s) Effect of reducing agents: For elucidating the role of disulfide bonds in the activity, 20 IRD proteins were treated with p-mercaptoethanol followed by heating. These preparations were checked for inhibitory activity by gel X-ray film contact print technique (GXCT) (Pichare & Kachole, 1994). For this, 0.5 HGPI units from each sample were separated on the 15% native-PAGE gel. After electrophoresis, gel was equilibrated with 0.1 M Tris-HCl buffer (pH 7.8) for 10 min followed by incubation in 25 0.04% trypsin for 10 min and Tris-HCl wash for 2 min. The gel was exposed to X-ray film for the time intervals of 5, 10 and 15 min, respectively. The films were washed 11 WO 2013/102937 PCT/IN2013/000011 with warm water and inhibitory activity bands were. visualized as unhydrolyzed gelatin on the X-ray film (Tamhane et al., 2005). Estimation of Free Thiol Content by Ellman's Assay: 2 jig of each protein was mixed in 100 pl of Tris-HCl buffer (pH -7.8); to this 50 d of Ellman's reagent and 840 pl of 5 MQ water were added. The mixture was incubated at 37 'C for 10 min and absorbance was measured at 412 nm. The concentration of free thiol content [RSH] of sample was calculated using the following equation (Aitken et al., 1996). AA412 E 412 TNB [RSH] where, AA 412 Afinar (3.1/3.2) (ADTNB - Abuffer) 10 and, E 4 12
    TNB
    2 - 1.415 x 104 cm'M' Effect of temperature: Each IRD protein (5 ptg) was heated from 60 to 100 'C for 15 min. The treated samples were then used for inhibition assay using BApNA and trypsin inhibition was estimated throughout the above mentioned range of temperature. Proteolytic stability and HGP inhibition visualization: To study the interaction and 15 stability of PIs with HGP in vitro, 0.5 HGPI units of individual IRDs (IRD-7, -9 and 12) were incubated with 0.5U HGP for two time points (5 min and 1 h) at 24 'C. These HGP- treated PIs were resolved on native-PAGE and processed for TI activity visualization as described above. This mixture of protease and PIs was also used for visualizing the remaining protease activity of HGP in the presence of inhibitor, on 8% 20 native-PAGE using GXCT. Combinatorial inhibition assay: In nature, PIs comprises of different combination of IRDs. IC 50 concentrations of each IRD(s) were used to formulate various combinations of IRDs to check their synergistic effect on HGP/bovine trypsin inhibition potential. The inhibition assay was carried out as already described. Four different formulations 25 i.e. IRD-7+9, IRD-7+12, IRD-9+12 and IRD-7+9+12, were used for inhibition assay. 12 WO 2013/102937 PCT/IN2013/000011 1.5 Molecular Dynamic (MD) Simulations of IRD-trypsin Complex The templates for molecular modeling, NMR structure of trypsin inhibitor from N. alata (PDB ID: 1TIHA; Figure SI) for IRD (Schirra et al., 2008) and the crystal structure of bovine trypsin (PDB ID: 3MI4; Figure SI) for HaTry (UniProt ID: B6CME9) were 5 selected based on sequence similarities. The 3D models were generated using MODELLER package (version 9.6). All the models were energy minimized using 1000 steps of the conjugate gradient algorithm and short MD simulations, as part of the MODELLER protocol in order to refine the side chain orientations. Fifty models were generated for each sequence, which were rated according to the GA341 and DOPE 10 scoring functions. The stereo-chemical properties of the final selected models were validated using PROCHECK and ProSA (https://prosa.services.came.sbg.ac.at/prosa.php) analyses. Structural superimposition of IRD-7, IRD-9 and IRD-12 with NaTI was also done using MODELLER and PyMol (The PyMol Molecular Graphics System, Version 1.2r3 pre, Schrodinger LLC) (Ghosh 15 et al., 2011). MODELLER software was used for in silico point mutation and three variants of IRD-9 namely IRD-9A, -9B and -9C were designed. These variants comprise mutations S7A, S8A in IRD-9A; C28S, C37S in -9B and S7A, S8A, C28A, C37A in -9C, respectively. Furthermore, each individual IRD was docked against H armigera trypsin and binding 20 energy (AGbind) was calculated for each complex. Nicotiana alata trypsin inhibitor (NaTI) was used as a control for correspondence with previous data (Schirra et al., 2010). The data obtained from binding energy calculation was normalized by AGbind of (NaTI+HaTry) complex. A heat map for this analysis was constructed with normalized AGbind values using TIGR Multi Experiment , Viewer (MeV, 25 http://www.tm4.org/mev.html). IRDs showing stronger binding with proteases and possessing large aa variation in reactive loop sequence were selected for further studies. The aa sequence analysis and multiple sequence alignment were performed using DNA star (Laser gene, DNASTAR, Madison, WI, USA) and Clustal X software. 13 WO 2013/102937 PCT/IN2013/000011 In order to obtain the structure of trypsin-IRD complex, protein-protein docking was carried out with the predicted models of HaTry and IRDs using the rigid-body docking program ZDOCK (version 3.0.1) (Chen et al., 2003). Six sets of protein-protein docking were carried out viz.HaTryIRD-7, HaTry_IRD-9, HaTryIRD-9A, HaTryIRD-9B, 5 HaTryIRD-9C and HaTryIRD-12. The binding site residues for HaTry and each of the IRDs were specified for docking, to allow the catalytic triad of HaTry (His69, Asp 114 and Ser2l 1) to interact with the reactive loop of the IRDs ("CPxNC"). After the initial docking, the best complex in each case was chosen based on the ZDOCK scores i.e. ZRANK, which is in the range of 15 to 31 for small proteins of 100 residues. 10 Explicit MD simulations were carried out for exploring the molecular mechanism of the dynamic interactions, the importance of the interacting residues in binding and the stability of the disulfide bridges. A set of six simulations was performed, corresponding to the six protease-IRD complexes using the GROMACS 4.0.7 package with GROMACS ffG43al force field for 20 ns each. All the six systems were solvated with 15 single-point charge (SPC) water model and neutralized with proper counter-ions. All the six systems were then energy minimized using 10,000 steps of the steepest descent. algorithm present in the GROMACS package followed by a 100 ps position restraining simulation - restraining the protein by a 1000 kJ/Mol harmonic constraint to relieve the close contacts with water molecules under NVT ensemble conditions. V-rescale 20 (modified Berendsen) temperature coupler was used to couple the temperature. Another equilibration run under NPT ensemble conditions was performed for 100 ps, before the final production run of 20 ns each for all the systems. V-rescale temperature and Parrinello-Rahman pressure couplers were used to maintain the temperature (293 K) and pressure (1 bar) values with the protein and non-protein (water and ions) molecules 25 separately coupled with a coupling constant of t = 0.1 picoseconds (ps). The isotropic pressure coupling was set with up = 2 ps. A time-step of 2 femtoseconds (fs) was used throughout with periodic boundary conditions and LINCS constraint algorithm was used to maintain the geometry of the molecules. van der Waal's interactions and Coulomb interactions were cutoff at 12 A with updates every 5 steps, while long range 14 WO 2013/102937 PCT/IN2013/000011 electrostatic interactions were calculated using the Particle-mesh Ewald (PME) method. All the simulations were performed on a PARAM Yuva cluster at the Centre for Development of Advanced Computing (C-DAC) at Pune, India, using 64 Intel Xeon 2.93 GHz Quad Core processors. The results were analysed using the in-built analysis 5 package of GROMACS, XMGRACE (http://plasmagate.weizmann.ac.il/Grace/) and in house developed scripts (Ghosh et al., 2011). The trajectories were visualized using VMD and all the images were rendered using PyMol. The overall stability of all the simulated systems was also checked with respect to temperature, pressure and potential energy. All the six simulated systems were in thermodynamic equilibrium during the 10 production simulation runs confirming the convergence of the individual trajectories (Figure S2). MD simulations were used to determine both the internal and the interaction potential for each complex. Free-energy of binding of the IRDs with the trypsin molecule was calculated by solving the linearized Generalized Born equation available with the 15 MM/GBSA module of AMBERI 1 package. The MM/GBSA module calculates the binding free energy (AGind) between a receptor (here, trypsin) and a ligand (here, IRD) to form a complex by solving the following equation, zIGb 1 d JH- TAS AdEMM +I AG,, 01 - ThIS AEMM +Einternai + AEeectrostatic+ JEydw 20 A Gsoi = GGB + AGSA where, AEinternai represents the internal energies due to bond stretching, angle bending and dihedral rotation, AEeecrostatic represents the electrostatic energy and JEdw represents the van der Waal's energy. JGGB represents the polar solvation energy using GB model and AGsA represents the non-polar solvation energy. For GB calculations, the 25 exterior dielectric constant was set at 80 and 1 was used for the solute dielectric constant. Pairwise GB model implemented in AMBER 11 was used for calculation, with parameters described by Tsui and Case (Tsui & Case, 2000). 15 WO 2013/102937 PCT/IN2013/000011 From the experimental inhibition concentrations IC 50 of three IRD(s) the experimental free energy of binding can be approximated using, AGbind = kB7K,= kBTn IC 50 + C where kB is the Boltzmann constant and T the absolute temperature. Here we have used 5 the Cheng-Prusoff s equation for competitive inhibition to convert an IC 50 value into an inhibition constant. In case of calculating relative free energy differences, C becomes zero. Calculated binding energy was correlated with experimental binding energy and regression coefficient (r 2 ) was calculated (Xu et al., 2012). 1.6 Modification of Serine Residues with phenylmethylsulfonyl fluoride (PMSF) 10 PMSF specifically binds irreversibly and covalently to serine and thus blocks its function. The reaction mixture containing inhibitor (IC 5 o concentration of each IRD in lml) in 100 mM Tris-HCl buffer, pH 7.8 and 5, 10 and 15 mM of PMSF was incubated at 30 'C for 1 hr. Aliquots were removed at different time intervals (15, 30, 45 and 60 min) and desalted thrice using disposable PD-10 Desalting Columns to remove the 15 excess PMSF from samples. Residual activities of these modified inhibitors were determined under standard assay conditions. Inhibitor sample incubated in the absence of PMSF served as control. Pseudo first-order plot for inactivation of IRDs by PMSF and second order plot of pseudo-first order rate constants (K, app) (min-) as a function of log of PMSF concentration were plotted using the equations from Koller et al. 1982 20 (Koller & Kolattukudy, 1982). Inhibitory activity of untreated and PMSF treated (10 mM for 2. hr at 30 'C) inhibitors were also assessed using GXCT method as described above. 1.7 Feeding Assay Bioassays were conducted by feeding H armigera larvae on artificial diet containing 25 PIs (Giri and Kachole, 1998). The artificial diet was prepared as reported (S. Nagarkatti, 1974). The artificial diet was supplemented with the PIs in appropriate quantities to give equal TI units (5 units/g of feed). The neonates that hatched from the eggs laid by the 16 WO 2013/102937 PCT/IN2013/000011 lab-reared second generation moths were reared for the first 3-4 days on control diet and then transferred to rCanPI-containing diets and control diet (artificial diet without PI) in separate sets of 25 larvae each; two replicates of each set were performed. Larval weights were meticulously recorded every alternate day and percent weight reduction in 5 the PI fed larvae was compared to that of the control group. The larval mortality and weights were recorded and compared with that of the control group to estimate the adverse effects of PIs on the growth and development of - armigera. 2. Results 2.1 Sequence and Structural Variation in IRDs 10 Phylogenetic (Figure IA) and multiple sequence alignment (Figure S3) analysis of CanPI IRDs showed significant divergence due to sequence variations in the reactive loop regions and in the number of cysteine residues. Heat map provides an overview of the binding energetics of all the 18 IRDs with target proteases. The data indicated that IRD-7, -9 and -. 12 bind more strongly to HaTry compared to the other IRDs and thus 15 selected for further analysis (Figure 1 B). The multiple sequence alignment of IRD-7, -9 and -12 with N. alata IRD (NaTI) showed over 90% sequence identity (Figure IC). In case of Pin-II PIs the major variation is found in the reactive loop (Kong & Ranganathan, 2008). The residues in the reactive loop of IRD-7 and -9 is "CPKNC", whereas in IRD-12 is "CPRNC". Another crucial variation is in the number of cysteine 20 residues present. The number of conserved cysteine residues in IRD-7 and -12 is eight while the same in IRD-9 is only six, making the latter one unique among IRDs. Two cysteines at 7 th and 8 th position of IRD-9 are replaced by serine residues, disrupting two disulfide bonds. PROCHECK analysis showed that the predicted models of IRDs and H armigera 25 trypsin had more than 95% residues in allowed and favored regions of the Ramachandran plot (Figure S4). ProSA analysis had also confirmed the quality of predicted models as good. In accordance with the structure of a typical IRD belonging to Pin-II PI family, the predicted structures also have three anti-parallel P sheets joined 17 WO 2013/102937 PCT/IN2013/000011 by disordered loops containing the reactive site and stabilized by four disulfide bonds (Figure 1D). It is thought that the disulfide bonds act as structural scaffold to hold the reactive site in a relatively rigid conformation and provide thermal and proteolytic stability (Bronsoms et al., 2011). A single 3 1 O-helix of one turn is also present in the 5 structure, the disordered loop is held by disulfide bond in IRD-7 and -12 whereas by a network of intra molecular hydrogen-bonds in IRD-9. Superposition of the predicted structures of IRD-7, -9 and -12 on the template structure of NaTI using Ca atom positions gave a root mean square deviation (RMSD)' of 1.1, 1.55 and 1.1 A, respectively. The central scaffold superposed well with the larger deviations confined to 10 solvent exposed surface loops. As expected the predicted structures of IRD-7 and -12 with eight cysteines, had four disulfide bonds (C4-C41, C7-C25, C8-C37 and. C14 C50), whereas IRD-9 with six cysteines had only two disulfide bonds (C4-C41, C14 C50) leaving two cysteine residues (C25 and C37) free. The predicted and validated H armigera trypsin (HaTry) model has classical trypsin-like fold consisting of two p 15 barrel domains and the juxtaposed catalytic residues. The catalytic triad in HaTry consists of the residues H69, D 114 and S211 (Figure S5). To assess the effect of aa variations on structural stability a 20 ns MD simulations was performed on IRD structures. The predicted structures remained stable throughout the 20ns simulation that was performed under NPT conditions at a temperature, of 293K 20 and 1 bar pressure. Post-simulation analysis of the intramolecular hydrogen bonds illustrated that IRD-9 with two disulfide bonds (C7-C25 and C8-C37) less, has a relatively higher density of intra-molecular hydrogen bonds as compared to IRD-7 and -12 (Figure ID and Table 2). These intramolecular hydrogen bonds might be substituting the two lost disulfide bonds of IRD-9 to stabilize the protein structure in 25 the active conformation and also might be protecting the molecules from a hydrophobic collapse (Hansen et al., 2007). The replaced serine residues in the place of two cysteines C7 and C8 in IRD-9 may be contributing to the increased number of hydrogen bonds. This might be a positive natural selection and led to functional differentiation of the inhibitor (Li et al., 2011). The relative orientations of the 18 WO 2013/102937 PCT/IN2013/000011 secondary-structural elements were conserved throughout the entire simulations with the RMSD values based on Ca positions remained below 2 A for IRD-9 and at about 3 A for IRD-7 and -12, while trypsin had an RMSD value of around 3 A with bound IRDs (Figure S6). 5 2.2 Inhibition Kinetics and Biochemical characterization of IRD-7, -9 and -12 IRD-7, -9 and -12 were extracellularly expressed in Pichia pastoris and the soluble fraction in each case yielded the single protein band in each case corresponding to ~5.8 kDa on 15% Tricine-SDS-PAGE (Figure 2A). Assays using BApNA and Azocasein as substrates showed that IRD-9 and -12 inhibited about 80 to 85% of HGP activity while 10 inhibition by IRD-7 was only 40 to 45% (Figure 2B). Both the substrates showed low inhibitory efficiency by IRD-7 and highest proficiency by IRD-9. Furthermore, the kinetic studies displayed a sigmoidal pattern with increasing concentrations of the inhibitors suggesting reversible and competitive inhibition with tight binding. IRD-9 turned out to be a stronger inhibitor of bovine trypsin (IC 50 15 -0.0022 mM) than IRD-7 (IC 50 -0.135 mM) and IRD-12 (IC 5 o ~0.065 mM) (Figure 2C). The inhibition constant Ki determined directly from IC 5 o by using the Cheng Prusoff's equation also confirmed the same (Table 1). Although the aa and structure variations of IRDs account for their differential binding efficiency, the exact molecular mechanism that contribute to binding efficiency is not understood. 20 It is known that the disulfide bonds are essential for the folding, function, and stability of IRDs (Schirra et al., 2010). In the present study the activity of all three IRDs seen on 15% Native-PAGE was lost in the reduced state (Figure 3A). Disulfide rich proteins are also known to show high thermal stability-(Bronsoms et al., 2011). Inhibition assays carried out at different temperatures showed that IRD-7 and -12 retained their 25 inhibitory activity against trypsin even at 90 0 C for 30 min whereas IRD-9 gradually lost activity starting from 70 "C (Figure 3B). The reduced thermal instability of IRD-9 might be due to decrease in the number of disulfide bonds. 19 WO 2013/102937 PCT/IN2013/000011 Interestingly, IRD-9 exhibited proteolytic resistance for 60 min when incubated with HGP as compared to IRD-7 and -12, both of which submitted to instantaneous proteolysis (Figure 3C). Gut extract of H armigera, a complex mixture of various trypsin and chymotrypsins like proteases, displayed at least 7 isoforms (HGP-1 to -7) 5 (Figure 3D). These isoforms of HGP vary in terms of properties and specificity. Interestingly, HGP isoforms were differentially inhibited by various IRDs. The activities of HGP-3 and -4 were inhibited by all IRDs, whereas that of HGP-5, -6 and -7 were inhibited exclusively by only IRD-9. Protease activity band between HGP-6 and 7 was developed only in the case of IRD-9 treatment and was not present even in 10 untreated HGP, indicating IRD-9 bound protease complex acquiring a different charge state. Thus, IRD-9 presented unique binding property and activity. The synergistic effect of IRDs was analysed by performing inhibition assay with combination of different IRDs in IC 50 concentration. The presence of IRD-9 in combination with IRD-7 and IRD-12 enhanced their corresponding HGPI activity from 15 49 to 65% and 51 to 63%, respectively (Figure 3E). Results obtained showed that IRD 9 might have a synergistic effect and can lead to higher potentiation of other IRDs. These biochemical evidences support the higher efficiency of varied combination of CanPIs/IRDs in inhibiting insect gut proteases, which signifies the biological relevance of sequence variation. 20 In silico studies indicated that IRD-9 has two free cysteine residues which may be in the form of thiol. This observation is confirmed by Ellman's assay, which estimates free thiol groups in small peptides. In the present study, it showed that 3.8 pM of IRD-9 had - 7.9 pM of free thiol (- 2 free cysteine residues) whereas a similar amount of IRD-7 and -12 had approximately -0.155 and -0.183 pM free thiol content (absence of 25 any free thiol). These results provided additional support to for the in silico predictions that IRD-7 and -12 had four disulfide bonds, whereas IRD-9 had only two leaving two remaining cysteines free. 20 WO 2013/102937 PCT/IN2013/000011 2.3 Molecular Mechanism of IRD(s)-HGP Interaction The 20 ns MD simulations were used to predict the binding affinities and hence the inhibitory effects of the individual IRDs against HaTry. The molecular models of the IRD bound HaTry predicted several atomic interactions with a reactive loop of 5 inhibitors that also explained the contribution of the solvent exposed reactive loop. In IRD-9-HaTry interaction, carbonyl oxygen atoms of MET-92, and SER-207 of HaTry active site formed hydrogen bonds with inhibitor side chain of LYS-39 and ASN-40, while side chains of MET-92, ASP- 192 and SER- 191 from HaTry form hydrogen bond with side chain of LYS-39, ASN-40 residues of IRD-9, respectively. ARG-39 from 10 IRD-12 reactive site formed three hydrogen bond between SER-207 and HIS-50 of the HaTry active site (Figure 4). In case of IRD-7, side chains of LYS-39 and PRO-38 residues of reactive loop form one hydrogen bond each, with carboxyl oxygen atom of HIS-50. There are additional hydrogen bond exist between side chain of CYS-37 form reactive loop of IRD-9 and -12, with carboxyl oxygen atom of ILE-210 and ARG-109 15 residue from HaTry. Although the interaction of active site of enzymes with all the three inhibitors were similar in nature, significant differences were observed in making the weak interaction like hydrogen bonding and van der Waal's interactions, which resulted in differential binding free energy of the complexes. IRD-9 forms the maximum nuriber of stable hydrogen bonds with the active site residues (HIS-50, ASP 20 95 and SER-207) of the HaTry and which were maintained for longer duration (Figure S7). Although IRD-12 forms relatively more hydrogen bonds, but they are very unstable as reflected by their fluctuating nature. MD simulations provides structural insight into an importance of inter/intra molecular hydrogen bonds and its effect on the interaction between protease and PIs. The results of this analysis were corroborated 25 with previous reports (Hansen et al., 2007). Post simulation analysis also explained experimentally observed increase in binding affinity, hence activity of IRD-9 towards proteases. 21 WO 2013/102937 PCT/IN2013/000011 Previous reports suggested.the role of C4-C41 disulfide bond in maintaining flexibility of the reactive loop and that of C8-C37 in holding a reactive loop of inhibitor in active and stable form. Interestingly in our study, IRD-9 was found to be a good inhibitor although it lacked a C8-C37 disulfide bond. In silico, analysis of a series of mutations 5 at the 7 th and the 8 th positions could provide insights into the significance of C7S and C8S variation on IRD-9 inhibitory activity. The three variants and the mutations tried are IRD-9A: S7A & S8A; IRD-9B: C28S & C37S and IRD-9C: S7A, S8A, C28A, and C37A. IRD-9A (~1 hydrogen bond) and -9C (~2 hydrogen bond) showed less number of intermolecular and intramolecular hydrogen bonds as compared to IRD-9B (-3 10 hydrogen bonds), in complex with HaTry. This analysis showed that replacement of cysteine with a hydrophilic residue, serine can prevent the hydrophobic collapse of the inhibitor molecule and might provide better flexibility and active conformation to the reactive loop and hence enhancement in the inhibitory potential (Schirra et al., 2010). Calculations of the free energy of binding between IRDs and HaTry (A Gbind) pointed to 15 a comparatively more stable complex formed by IRD-9 with the lowest AG value of 68.63 Kcal/mol, as compared to IRD-7 (-40.03 Kcal/mol) and IRD-12 (-54.01 Kcal/mol), a trend similar to what observed in inhibition assays. The free energy of binding was also calculated for HaTryIRD-9 variants complexes, in which the binding of IRD-9B (-74.14 Kcal/mol)-was found more stable as compared to IRD-9A (-39.88; 20 Kcal/mol) and IRD-9C (-38.04 kcal/mol), respectively. This analysis of the variants has provided valuable insight for carrying out potential site directed mutations of IRDs for higher stability and adaptability. There was good correlation between theoretically calculated and experimentally found AG values (Table 1). The high correlation coefficient (r 2 =0.97) between-the calculated and the experimentally determined binding 25 free energies supports our observation (Figure 5). This implies the reliability of the predicted binding conformations and interaction of the inhibitors with HaTry. The higher conformational flexibility of IRD-9 by the loss of two disulfide bonds has helped it to spatially adapt a better complementary shape suited to the active site of HaTry compared to the more rigid four disulfide containing IRD-7 and IRD-12. 22 WO 2013/102937 PCT/IN2013/000011 2.4 Effect of Serine Residues modification in inhibition potential of IRDs The effect of PMSF on the activity of IRDs is shown in the Figure 6. Reaction of the inhibitor with PMSF leads to modification of one serine residue (number of residues modified were deduced from graph of Log Kapp against conc. of PMSF) in IRD-9 and 5 resulted in 35 to 45% activity loss. Modification of IRD-7 and -12 did not show significant effect on the activity (Figure 6A). Furthermore, activity visualization assay showed that PMSF modified IRD-9 has reduced inhibition potential as compared to modified. IRD-7 and -12 (Figure 6B). These results pointed out that, serine could be involved in holding the reactive loop in proper position through a network of hydrogen 10 bonds which was blocked on treatment with PMSF and resulted in loss of inhibitory activity of IRD-9. Thus, result indicated that serine residues were not directly involved in the interaction, but they significantly affect the binding of inhibitor with a protease molecule. 2.5 Ingestion of IRD-9 Inhibits the Development of H.armigera Larvae. 15 The discovery that IRD-9 abolished the gut proteases activity led us to investigate whether the IRD-9 have a more marked effect on insect growth and development than other IRDs. To test this possibility, we placed H armigera neonates on artificial diets with and without added PIs and recorded weight gain on days 2, 4, 6, 8 and 10 (Fig. 7A and 7B). At day 10, larvae fed diets containing IRD-9 weighed significantly less 20 than control diet fed larvae as well as larvae fed on IRD-7 and IRD-12. Furthermore, larvae fed on IRD9 shows relatively higher mortality rate as compared to larvae fed on control, IRD-7 and IRD-12 containing diets (Fig 7C). In sum, this study employed a combination of-experimental and theoretical approaches to investigate the molecular details of HaTry-IRD interaction. Expression and 25 biochemical characterization of IRD-7, -9 and -12 revealed IRD's sequence-dependent variation of inhibition. Furthermore, IRD-9 lacking two disulfide bonds shows phenomenal inhibition activity compared to other IRDs. This natural variant also exhibit special attributes like stability to proteolysis and inhibitory synergistic effect on 23 WO 2013/102937 PCT/IN2013/000011 other IRDs etc., which makes this molecule unique among the members of Pin-Il inhibitor family. Explicit MD simulation of protease-inhibitor complex suggests. that the loss of disulfide bonds in IRD-9 might be compensated by higher density of intramolecular hydrogen bonds and reactive loop flexibility to bind tightly to target 5 proteases. Chemical modification studies of serine residues combined with MD simulation confirms the role of serine residues that replaced cysteine in increasing the inhibition potential of IRD-9. In vitro efficacy of IRD-9 also reflects as In vivo efficiency and thus led to significant impairment of H. armigera larvae fed on it. This insight into interaction mechanisms and role of certain residue changes in unexpected 10 increase of IRD inhibition potential will lead to development of new range of potent PIs for effective pest management strategies. References: 1. Aitken, A., Learmonth, M., (1996).Estimation of Disulfide Bonds Using Ellman's Reagent. The Protein Protocols Handbook V, 487-488. 15 2. Antcheva, N., Pintar, A., Patthy, A., Simoncsits, A., Barta, E., Tchorbanov, B., Pongor, S. (2001). Proteins of circularly permuted sequence present within the same organism, the major serine proteinase inhibitor from Capsicum annuumseeds. Protein Sci., 10, 2280-2290. 3. 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Plant Physiol., 134, 1181-1190. 25 29 WO 2013/102937 PCT/IN2013/000011 SEQUENCE LISTING <110> CSIR,IN <120> A NOVEL PLANT PROTEINASE INHIBITOR AGAINST INSECTS GUT PROTEASES 5 <130> 0035DEL2012 <160> 3 <170> PatentIn version 3.5 <210> 1 <211> 50 0 <212> PRT <213> Capsicum annuum IRD 7 <400> 1 Glu Pro Ile Cys Thr Asn Cys Cys Ala Gly Leu Lys Gly Cys Asn Tyr 5 1 5 10 15 Tyr Asn Ala Asp Gly Thr Phe Ile Cys Glu Gly Glu Ser Asp Pro Asn 20 25 30 20 His Pro Lys Ala Cys Pro Lys Asn Cys Asp Pro Asn Ile Ala Tyr Ser 35 40 45 25 Leu Cys 50 30 WO 2013/102937 PCT/IN2013/000011 <210> 2 <211> 50 <212> PRT <213> Capsicum annuum IRD 9 5 <400> 2 Gln Pro Ile Cys Thr Asn Ser Ser Ala Gly Leu Lys Gly Cys Asn Tyr 1 5 10 15 10 Tyr Asn Ala Asp Gly Thr Phe Ile Cys Glu Gly Glu Ser Asp Pro Asn 20 25 30 15 His Pro Lys Ala Cys Pro Lys Asn Cys Asp Pro Asn Ile Ala Tyr Ser 35 40 45 20 Leu Cys 50 25 31 WO 2013/102937 PCT/IN2013/000011 <210> 3 <211> 50 <212> PRT <213> Capsicum annuum IRD 12 5 <400> 3 Asn Arg Leu Cys Thr Asn Cys Cys Ala Gly Arg Lys Gly Cys Asn Tyr 1 5 10 15 10 Tyr Ser Ala Asp Gly Thr Phe Ile Cys Glu Gly Glu Ser Asp Pro Asn 20 25 30 15 Asn Pro Lys Ala Cys Pro Arg Asn Cys Asp Pro Asn Ile Ala Tyr Ser 35 40 45 20 Leu Cys 50 25 32
    权利要求:
    Claims (3)
    [1] 1. A method for effective management of Helicoverpa armigera using inhibitory repeat domain IRD-9, a Pin-II type proteinase inhibitor (PIs) comprising the steps: 5 a. providing IRD -9 having Seq Id no.
    [2] 2 from non-host plant Capsicum annuum, b. cloning of IRD-9 in Pichiapastoris (Yeast expression system) in pPIC-9 vector, c. expressing and purifying IRD-9 protein, d. feeding IRD-9 protein to Helicoverpa armigera in artificial diet, e. calculating growth parameters for antibiosis effect of IRD-9. 10 2. The method as claimed in claim 1, wherein IRD-9 of the Pin-II family proteinase inhibitors (CanPIs) is characterized in having: i. Molecular Weight: 5.8 Kd ii. Sequence length: 50 amino acids iii. No. of cysteine residues: 6 15 iv. No. of disulfide bond: 2 v. Inhibition constant (Ki): ~0.0022 mM vi. Molecular interaction: reactive loop of IRD-9 form multiple hydrogen bonding with active site of target proteases.
    [3] 3. Use of IRD-9 a variant unit of the Pin-Il family proteinase inhibitors (CanPIs) as 20 claimed in claim 1, for effective pest management including efficient antibiosis of hazardous agricultural pest. 33
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    同族专利:
    公开号 | 公开日
    WO2013102937A3|2013-10-31|
    WO2013102937A2|2013-07-11|
    US9357777B2|2016-06-07|
    US20150087584A1|2015-03-26|
    AU2013207052B2|2016-09-29|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US9357777B2|2012-01-05|2016-06-07|Council Of Scientific And Industrial Research|Method for effective management of helicoverpa armigera|US9357777B2|2012-01-05|2016-06-07|Council Of Scientific And Industrial Research|Method for effective management of helicoverpa armigera|
    法律状态:
    2017-02-02| FGA| Letters patent sealed or granted (standard patent)|
    优先权:
    申请号 | 申请日 | 专利标题
    IN35DE2012||2012-01-05||
    IN0035/DEL/2012||2012-01-05||
    PCT/IN2013/000011|WO2013102937A2|2012-01-05|2013-01-07|Method for effective management of helicoverpa armigera|
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